Table 1. Primer list.
| Name | Sequence | For HBV, position relative to the EcoRI site | Usage |
|---|---|---|---|
| HBV RNA 1 F | TGTCAACACTAATATGGGCCTAA | 2164–2186 | HBV 3.5 kb RNA (RT-qPCR) |
| HBV RNA 1 R | AGGGGCATTTGGTGGTCTAT | 2295–2314 | |
| HBV RNA 2 F | ACAAGGTAGGAGCTGGAGCA | 2981–3000 | HBV 3.5 kb and 2.4 kb RNAs (RT-qPCR) |
| HBV RNA 2 R | GTAGGCTGCCTTCCTGACTG | 3112–3131 | |
| HBV RNA 3 F | GCTTTCACTTTCTCGCCAAC | 1087–1106 | HBV 3.5 kb, 2.4 kb and 2.1 kb RNAs (RT-qPCR) |
| HBV RNA 3 R | GAGTTCCGCAGTATGGATCG | 1262–1281 | |
| HBV RNA 4 F | GTGCACTTCGCTTCACCTCT | 1579–1598 | total HBV RNAs (RT-qPCR) |
| HBV RNA 4 R | AGCTTGGAGGCTTGAACAGT | 1859–1878 | |
| hSAMHD1_F | TCACAGGCGCATTACTGCC | SAMHD1 mRNAs (RT-qPCR) | |
| hSAMHD1_R | GGATTTGAACCAATCGCTGGA | ||
| HBV RC F | CAC TCT ATG GAA GGC GGG TA | 2753–2772 | HBV extracellular DNA (qPCR) |
| HBV RC R | TGC TCC AGC TCC TAC CTT GT | 2981–3000 | |
| HBV-total-DNA-F | GTTGCCCGTTTGTCCTCTAATTC | 465–487 | intracellular or cytoplasmic HBV DNA (qPCR) |
| HBV-total-DNA-R | GGAGGGATACATAGAGGTTCCTTGA | 540–564 | |
| β-globin-F | CAGGTACGGCTGTCATCACTTAGA | normalisation of qPCR | |
| β-globin-R | CATGGTGTCTGTTTGAGGTTGCTA | ||
| RPL13A_F | CCT GGA GGA GAA GAG GAA AGA GA | normalisation of RT-qPCR | |
| RPL13A_R | TTG AGG ACC TCT GTG TAT TTG TCA A |