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. Author manuscript; available in PMC: 2016 May 27.
Published in final edited form as: Contrast Media Mol Imaging. 2012 Jan-Feb;7(1):26–34. doi: 10.1002/cmmi.460

Figure 6.

Figure 6

The dependence of CEST on concentration. (A) The percentage CEST effects of the amide (solid circles) and amine (open circles) of Yb– DO3A–oAA were measured at pH 7.20 and 37°C using 10 µT saturation power over a range of concentrations. The curved lines were calculated from the Hanes-like analysis shown in (B) [see (39) for details]. The ratio of the CEST effects (diamonds) was fitted with a linear function to demonstrate that the ratio is independent of concentration. (B) The high R2 correlation coefficients of the linear fit of the Hanes-like analysis method to the experimental data indicated that the chemical exchange of the amide (solid circles) or amine (open circles) with water can be approximated as a two-pool model. (C) The pH values measured from the CEST effects of Yb–DO3A–oAA were independent of concentration. The dashed line represents the pH measured with an electrode.