Figure 5. AMPK inhibition decreases tau phosphorylation in neurons.

Primary neurons at 15 DIV were treated for the indicated times with the AMPK inhibitor Compound C (10 μM). WB analysis (a) and quantification of total tau (b) and phosphorylated tau at epitopes Ser^262/356 (c), Thr²³¹ (d), Ser^396/404 (e) and Ser²⁰² (f) expressed in ratios. Cytotoxicity was assessed after 6 h and 24 h of Compound C treatment using LDH assay (g). Results represent mean ± SD, n = 4–6. a.u., arbitrary units. *p < 0.05, **p < 0.01, ***p < 0.001 compared to Ctrl, One way ANOVA with Bonferroni’s post hoc test.