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. 2015 Jul 17;159(1):87–99. doi: 10.1093/jb/mvv073

Fig. 1.

Fig. 1

Expression and purification of recombinant PqqE. (A) SDS–PAGE analysis of cell debris (Deb) and lysate (Lys) of E. coli Rosetta 2 (DE3) cells expressing wild-type (WT) and C32S mutant (C32S) of PqqE from M.extorquens AM1. Fifteen microliters of cell lysate and debris (dissolved in 5 µl of sample buffer) were applied in each well of a 10% (w/v) gel. A triangle indicates the protein band corresponding to the expressed PqqE-N. (B) SDS–PAGE analysis of the purified wild-type (WT) and C32S mutant (C32S) of PqqE-N (5 µg protein/well). M: PageRuler marker (Thermo Scientific, Waltham, MA).