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SAM cleavage activities and Fe/S contents
| PqqE-N | As-purified |
Reconstituted |
||||
|---|---|---|---|---|---|---|
| SAM cleavage activity (nmol/min/mg) | Fe/PqqE-N (mol/mol) | S/PqqE-N (mol/mol) | SAM cleavage activity (nmol/min/mg) | Fe/PqqE-N (mol/mol) | S/PqqE-N (mol/mol) | |
| Wild-type | 0.418 ± 0.175 (n = 30) | 5.36 ± 0.11 (n = 7) | 8.75 ± 0.21 (n = 7) | 0.609 ± 0.156 (n = 29) | 12.7 ± 1.0 (n = 7) | 16.4 ± 1.3 (n = 7) |
| C32S mutant | 0 | 3.84 ± 0.65 (n = 7) | 5.30 ± 0.33 (n = 7) | 0 | 10.5 ± 1.3 (n = 7) | 12.1 ± 1.5 (n = 7) |
