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. 2015 Sep 2;159(1):123–132. doi: 10.1093/jb/mvv083

Table I.

The number of molecules of each αEGFR[scFv] fusion protein per cell in each fraction, as estimated by quantitative immunoblotting with anti-FLAG IgG.

Mean ± SD (105 molecules/cell)
c n
αEGFR[scFv] 25.0 ± 10.8 4.7 ± 2.5
αEGFR[scFv]-NLS 14.4 ± 6.1 3.5 ± 2.0
αEGFR[scFv]-B18 14.8 ± 5.2 1.2 ± 0.6
αEGFR[scFv]-B18-NLS 8.3 ± 3.6 1.6 ± 0.6
αEGFR[scFv]-B55 11.6 ± 3.2 1.2 ± 0.5
αEGFR[scFv]-B55-NLS 12.4 ± 5.0 1.8 ± 0.8
αEGFR[scFv]-TAT 18.6 ± 8.3 2.4 ± 1.0
αEGFR[scFv]-TAT-NLS 13.4 ± 5.4 2.6 ± 1.0

c, the cell lysate excluding nuclear proteins (non-nuclear fraction); n, nuclear fraction. N = 5.