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Labeling of multiple α-glucosidases and their identification by proteomics. (a) Labeling of proteins in fibroblast lysate with compound 5. (b) Workflow of pull-down proteomics experiments. (c) In-gel digestion silver staining and identification of target proteins modified by biotin-ABP 6. (d) Glycosidase identification with Mascot score and emPAI values after on-bead pull-down and processing of fibroblast lysate with biotin-ABP 6.
