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In vitro ABP labeling and Western blot detection of α-glucosidases in wild type and Pompe fibroblasts. (a). In vitro GAA labeling at pH 4.0 with 3, followed by Western blot detection of GAA in various fibroblast lysates, containing wild type or mutant (Pompe) GAA. (b). In vitro GANAB labeling at pH 7.0 with 3 followed by Western blot detection of GAA in various fibroblast lysates, containing wild type or mutant (Pompe) GAA.
