Table 4.
Annealing sites in primers for amplification of integration cassettes are shown in red, while overhangs for homologues recombination have been marker blue. For cloning primers the restriction sites are indicated in bold letters
Annealing sites in primers for amplification of integration cassettes are shown in red, while overhangs for homologues recombination have been marker blue. For cloning primers the restriction sites are indicated in bold letters