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. 2016 May 26;16:6. doi: 10.1186/s12895-016-0043-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Exposure to UVB plus heat induced a significant decrease in acetylated p53 and an increase in SIRT1 protein levels in NHEK and in skin models. a Immunohistochemical staining of CPD (red), p53-a382 or SIRT1-p (green) and DAPI (blue) in untreated or UVB and/or heat treated ex vivo skin keratinocytes. Inset images are an enlarged view of SIRT1/DNA damaged (CPD positive) keratinocytes, which are also indicated by red arrows. Broken lines denote the epidermal/dermal border. Scale bar (white line) =100 μm. b–e Bar graphs of mean ± SD percent keratinocytes with CPD and positive for (b and c) p53-a382 or (d and e) SIRT1-p per field of view in (b and d) primary NHEK or (c and e) in skin models. Statistically significant differences are indicated with * for p-values <0.05