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. 2016 May 26;16:111. doi: 10.1186/s12872-016-0280-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Agarose gel electrophoresis image of restriction fragment length polymorphism. Amplified DNA by PCR was digested with the restriction enzyme 12 U of ApaI overnight at 37 °C and resolved in 2 % agarose gel stained with ethidium bromide at 50 V for 2 h. Resolved band patterns were photographed under ultra violet light by the Elite camera systems