Table 1.
Primers employed for A. marginale identification and characterization
| Gene | Product | Primer sequence | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| msp5 | major surface protein MSP5 | F: 5' GCATAGCCTCCCCCTCTTTC 3' | 548 | [9] |
| R: 5' TCCTCGCCTTGCCCCTCAGA 3' | ||||
| F: 5' TACACGTGCCCTACCGACTTA 3' | 345 | |||
| R: 5' TCCTCGCCTTGCCCCTCAGA 3' | ||||
| msp1α | major surface protein MSP1a | F: 5'GCATTACAACGCAACGCTTGAG 3' | 84–87 bp tandem repeats | [10] |
| R: 5'GCTTTACGCCGCCGCCTGCGCC 3' | ||||
| dnaA | Chromosomal replication initiation protein | F: 5' GTTCATAAGCGGGAAGGACA 3' | 512 | [11] |
| R: 5' CTTGTCTCGGTCTGGCTAGG 3' | ||||
| ftsZ | Cell division protein FtsZ | F: 5' CCTGACCACCAATCCGTATC 3' | 575 | |
| R: 5' CCCGTATGAAGCACCGTATC 3' | ||||
| groEl | Chaperonin GroEL | F: 5' AGCATAAAGCCCGAGGAACCTT 3' | 699 | |
| R: 5' GCCGAGCATGTCCTTCCTTCTG 3' | ||||
| lipA | Lipoyl synthase | F: 5' TGTGGATAGGGACGACCTTC 3' | 538 | |
| R: 5' AAAGTCATCCTCAGCGTGGT 3' | ||||
| recA | Recombinase A | F: 5' GGGCGGTAACTGTGCTTTTA 3' | 579 | |
| R: 5' ACGCCCATGTCGACTATCTC 3' | ||||
| secY | Preprotein translocase subunit SecY | F: 5' TTCACGCTGCTAGCCCTAAT 3' | 501 | |
| R: 5' TACGAGGGAAATGCCGTTAC 3' | ||||
| sucB | Dihydrolipoamide acetyltransferase component | F: 5' GAGATAGCATCTCCGGTTGC 3' | 808 | |
| R: 5' CTCCCCTGGCCTTTTTACTC 3' |