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Background levels of bacterial genomes based on 16S rRNA gene universal primers suggest a level of at least 4 bacterial genomes per µl of qPCR reaction mixture. Although the true amount of contaminating bacterial DNA would be 4 + NTC, assuming the lowest possible figure, a standard 50 µl qPCR reaction would contain at least 200 E. coli-equivalent genomes or approximately 1400 rRNA gene copies contaminating the reaction
