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. 2016 May 27;17:64. doi: 10.1186/s12931-016-0377-9

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© Zhang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Representative Quality metrics of the targeted-NGS method and strategy for mutation screening in the targeted genes. a Representation of the average depth of each sample. The coverage depth was >200× for each sample. b Mean coverage obtained for the different genes across patients. Bar chart displaying the mean coverage of each gene in all samples. Error bars represent standard deviation. The blue line shows the lower limit of coverage appropriate to perform CNV analysis (30×). c Strategy for comprehensive mutation screening of the targeted genes in PSP cohort