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. 2016 May 27;17:64. doi: 10.1186/s12931-016-0377-9

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© Zhang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Sanger sequencing and ESEfinder results of the branch-site mutation. a Sequencing chromatograms show a heterozygous mutation c.1177–21G > A in FLCN gene. The arrow indicates the position of the nucleotide mutation and the boxed nucleotides indicate the branch site predicted by ESEfinder. b ESEfinder results for the c.1177–21G > A substitution and for the wild type of the branch-site sequence