Table 2.

General molecular characteristic of the samples investigated.

Sample ID	Depth (mbsf)	Unit	16S rRNA copies (× 104)	% Bacteria		Reads after filtering	OTU #	Shared with sediment
				qPCR	Amplicon			OTUs	Reads of total %
U1382A
2R_1C	110	I	5.7	98	100	8,598	371	198	72
3R_2B	115	I	8.3	100	100	12,966	174	114	81
3R_3A	117	I	2.7	100	99	16,574	96	66	80
3R_4B	117	I	2.4	100	100	11,349	224	133	81
4R_1B	123	II	4.8	96	97	15,330	215	128	78
5R_1B	133	II	3.9	92	99	19,517	279	168	83
6R_1A	142	II	2.1	100	100	20,437	175	101	69
7R_2B	153	IV	3.8	100	100	10,968	193	127	85
8R_1A	161	V	1.8	100	100	23,251	42	23	80
8R_1B	161	V	4.1	95	100	12,495	261	142	80
8R_2F	162	V	0.3	100	100	19,858	16	10	99
8R_3G	163	V	0.5	100	100	19,145	25	18	22
8R_4D	163	V	0.6	100	86	25,028	27	20	31
9R_1C	172	V	4.2	92	99	16,873	314	185	82
10R_3D	183	VI	5.8	97	99	18,080	283	148	79
12R_1A	199	VII	3.0	92	98	19,506	273	153	75
U1383C
2R_2E	72	I	2.8	99	100	19,163	319	165	63
3R_1B	77	I	3.1	98	100	18,240	141	75	75
4R_1B	87	I	1.9	98	97	19,144	124	72	72
5R_1BI	97	I	3.1	91	96	15,164	177	124	77
5R_1BII	97	I	2.6	99	99	19,553	330	168	75
6R_1A	105	I	2.2	100	100	23,627	243	146	71
10R_1A	144	II	2.1	90	100	25,841	158	99	86
10R_1D	145	II	2.9	98	100	17,447	326	161	83
11R_1C	154	II	1.7	99	100	24,686	204	116	87
19R_1B	212	III	3.0	98	100	19,107	264	130	84
19R_1A	212	III	3.3	99	100	18,946	126	81	88
20R_1A	219	III	2.9	100	100	3916	148	90	81
24R_1B	257	III	1.9	100	100	12,775	162	99	86
24R_1A	256	III	3.2	100	100	20,900	213	121	86
27R_1A	285	III	3.9	99	100	9,811	162	113	87
29R_1A	300	III	0.6	100	98	11,218	69	48	82
30R_1A	304	III	3.3	100	100	22,903	229	117	88

Including gene copy numbers of total prokaryotic 16S rRNA gene copies per gram of sample material. Percent of the total community related to the domain Bacteria reported both from the qPCR and the amplicon library. The total number of reads after filtering. Number of OTUs in each sample (97% similarity). The number of OTUs and the % of total read that is shared with the samples in the above sediment from North Pond (1383E).