Figure 4.

Functional activity of TRP channels in cells infected by corresponding shRNAs. (A) Probenecid‐induced Ca²⁺ _i increase in cells infected by TRPV2 shRNA or scrambled shRNA. (B) Mean values of Ca²⁺ _i increase induced by the TRPV2 activator probenecid (1 mM) in cells infected by TRPV2 shRNA (n = 45) or scrambled shRNA (n = 42, **P < 0.01 versus scrambled shRNA). (C) 4α‐PDD‐induced Ca²⁺ _i oscillations in cells infected by scrambled shRNA or TRPV4 shRNA. (D) Mean percentage of cells with Ca²⁺ _i oscillations in the presence of the TRPV4 activator 4α‐PDD (10 μM) (n = 62, **P < 0.01 versus scrambled shRNA, n = 45). TRPV, transient receptor potential vanilloid.