Skip to main content
. 2016 Jan 15;20(6):1062–1075. doi: 10.1111/jcmm.12761

Figure 3.

Figure 3

PI3K inhibitor LY294002 attenuates the protective effect of EGF on BSCB after SCI. (A) Representative whole spinal cords show that Evan's Blue dye permeabilized into injury spinal cord at 1 day (n = 4/group). (B) Quantification of the amount of Evan's Blue at 1 day (μg/g). (C) Representative confocal images of Evans Blue Dye extravasation at 1 day of Sham, SCI group, SCI rat treated with EGF group and SCI rat treated with EGF and LY294002 group. (D) Protein expressions of p120‐Catenin, β‐Catenin, Occludin, Claudin‐5, p‐Akt, Akt, GTP‐Rac1 and Total‐Rac1 in the spinal cord segment at the contusion epicentre. (E and F) The optical density analysis of p120‐Catenin, β‐Catenin, Occludin and Claudin‐5 protein. (G and H) The optical density analysis of p‐Akt/Akt and GTP‐Rac1/Total‐Rac1 proteins. *represents P < 0.05 versus the Sham group, #represents P < 0.05 versus the SCI group, & represents P < 0.05 versus the SCI+EGF group, n = 5.