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. 2016 Jan 15;20(6):1062–1075. doi: 10.1111/jcmm.12761

Figure 4.

Figure 4

PI3K inhibitor LY294002 attenuates the protective effect that EGF inhibits the loss of TJ and AJ in Human brain microvascular endothelial cells after OGD. (A) Evaluation of paracellular permeability by FITC–dextran. (B) Protein expressions of p120‐Catenin, β‐Catenin, Occludin, Claudin‐5, p‐Akt, Akt, GTP‐Rac1 and Total‐Rac1. (C and D) The optical density analysis of p120‐Catenin, β‐Catenin, Occludin and Claudin‐5 protein. (E and F) The optical density analysis of p‐Akt/Akt and GTP‐Rac1/Total‐Rac1 proteins. (G and H) Immunofluorescence staining of TJ and AJ protein. Nucleus (blue) was labelled with Hoechst. *represents P < 0.05 versus the Control group, #represents P < 0.05 versus the OGD group, & represents P < 0.05 versus the OGD + EGF group.