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. Author manuscript; available in PMC: 2016 May 27.
Published in final edited form as: Can J Physiol Pharmacol. 2014 Jan 31;92(7):598–604. doi: 10.1139/cjpp-2013-0490

Figure 4. High glucose transiently inhibits basal ERK 1/2 phosphorylation.

Figure 4

Cardiac fibroblasts were cultured in either low glucose (5 mM) or high glucose (25 mM) for the designated time and cell lysates were collected and subjected to Western blot analysis for phospho-ERK and total ERK. A) Basal ERK phosphorylation was lower in fibroblasts treated with high glucose for 20 min (0.13±0.06 fold, p<0.05), 60 min (0.08±0.42 fold, p<0.001), and 4 h (0.20±0.16 fold, p<0.05). Data are representative of duplicate wells from 4 separate CF preparations (n=4). Statistical significance (p<0.05) between groups was determined by one-way ANOVA.