SecA-signal peptide chimeras used in the study. The name of each protein chimera is given on the left with a pictorial diagram of the chimera given on the right. bsSecA or ecSecA refers to SecA proteins from B. subtilis or E. coli, respectively, and numbers indicate the residues included in each fusion. PhoAss, LamBss, or KRRLamBss refers to signal peptides derived from E. coli alkaline phosphatase, lambda receptor, or a KRR-variant of the lambda receptor protein, respectively. GS(2.5) or GS(5) refers to glycine-serine linkers with 2.5 or 5 repetitions, respectively (Supporting Information, Table S1). The carboxyl-terminal hexa-histidine tag present on each chimera is not shown. Regions are not drawn to scale.