Table 5.

Primers for InDel markers and SNP markers developed

Primer	Forward (5’-3’)	Reverse (5’-3’)	Product length (bp)	Annealing temperature (°C)
INDEL7-1	tcgataaaagttcagtttgacggc	actttttcatccgcgacgaatatc	68 (62)*	55
INDEL7-2	tgaagtggcatgatccatctacac	tgtactgcactgcagtggatgc	81 (75)*	55
INDEL7-3	tttttagattatttacttcacg	taatcaagaaggacttttgag	65 (69)*	50
SNP7-1	tcggattcaatgtgtcactctc	acatgctactagttattcctcgtaaac	111	58
SNP7-2	tgacgcattctcgatggagtc	tatcggggacttgttctcattc	80	58
SNP7-3	aggaataccagatgctgttgtcg	aactccccctccagtgtagcc	78	60
SNP7-4	tcaaagacatgacatcacgacac	cagagcacctataagtaacagtctaac	84	58
SNP7-5	tcattagcacatatttattgtagcacc	gaaaaaaccaattacacagattgc	106	60

*Number in brackets indicates the product length of PA64s.