Table 2.

Bi-directional SNP primer sequences of OsFRO1

Primer name*	Sequence 5’ → 3’
OsFRO1_Ex.4F	GGTGGATGAAGACACTACTGC
OsFRO1_Ex.4R	CACAGGACATTGGTCATAGCA
OsFRO1_Ex.4_SAP_A	GGCCTCCGGTTCGGATCGA
OsFRO1_Ex.4_SAP_G	GCCATGCAAAACAACCCGAC
OsFRO1_Ex.5F	TCATCTACTCTGTTTTGGAGGT
OsFRO1_Ex.5R	CTTGCTGGCTTTGAGAAGACT
OsFRO1_Ex.5_SAP_G	TTCCTGAGGTTCTGGCAATG
OsFRO1_Ex.5_SAP_C	TGTCCACCTTGGCCCTGG

*Each SAP primer set was amplified using the KAPA 2 G Robust HS protocol (KAPABIOSYSTEMS, Woburn, USA) under the following thermal cycling conditions: one cycle at 95°C for 3 min; 35 cycles of 30 s denaturation at 95°C, 30 s annealing at 61°C, and 30 s extension at 72°C; and a final extension at 72°C for 2 min.