Table 2.
Frequency of Occurrence and Relative Amount of Fn-Fibrin Complexes in Plasma of Patients with Atherosclerosis
| Plasma FN forms | M m (kDa) | Frequency of occurrence and relative amount of FN forms in plasma groups 1–3 Mean value of relative amount ± SD |
||
|---|---|---|---|---|
| 1. Significant coronary artery changes n = 66 | 2. Mild coronary artery changes n = 14 | 3. Age-matched normal group n = 21 | ||
| FN monomer ± degradations fragments | ∼250 | 0.94 (62) 6.24 ± 3.4 |
0.92 (14) 6.0 ± 3.2 |
0.85 (18) 4.95 ± 4.5 |
| FN dimer | ∼500 | 1 (66) 74.66 ± 17.7 |
1 (14) 86.33 ± 8.5 |
1 (21) 88.13 ± 7.5 |
| FN-fibrin complexes | I ∼750 |
0.83 (55) 13.36 ± 10.2 P < 0.048 |
0.64 (9) 7.25 ± 6.7 |
0.76 (16) 6.88 ± 4.6 |
| II ∼1000 |
0.39 (26) 2.95 ± 4.6 P < 0.019 |
0.07 (1) 0.19 ± 0.7 a2.7 |
Not detected |
|
| III ∼1300 |
0.28 (19) 1.62 ± 3.3 |
Not Detected |
Not Detected |
|
| IV ∼1600 |
0.2 (13) 0.77 ± 2.1 |
Not detected |
Not detected |
|
| V ∼1900 |
0.05 (3) 0.21 ± 1.0 |
Not detected |
Not detected |
|
| VI ∼2200 |
0.03 (2) 0.08 ± 0.6 |
Not detected |
Not detected |
|
Plasma FN forms were revealed by SDS-agarose immunoblotting (see Fig. 2). Frequency of occurrence is the ratio of the number of samples containing the FN form to the total number of samples. In parentheses are given the number of samples which revealed the respective FN band. The relative amount of the FN band is the percentage of the total number of pixels found in the electrophoresis path and is expressed as mean value ± SD. Data are presented as mean values ± SD. Significantly different from the age-matched normal group calculated by the Kruskal–Wallis and post hoc tests
aThe relative amount for the sample from group 2 with mild coronary artery changes (∼1000 kDa)