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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Nature. 2016 May 18;533(7604):552–556. doi: 10.1038/nature17979

Extended Data Fig. 10. Memory CD4+ T cells are required for the increase in antibody levels and vascular permeability in the brain following VSV immunization and challenge.

Extended Data Fig. 10

(a) C57/BL6 mice were immunized i.v. with WT VSV (2 × 106 pfu/mouse). Five weeks later, these mice were challenged intranasally with WT VSV (1 × 107 pfu/mouse). Six days after challenge, VSV-specific IFN-γ+ CD4+ T cells in spleen (CD45.2+) following co-culture with HI-VSV loaded splenocytes (CD45.1+) or HI HSV-2 loaded splenocytes were analyzed by flow cytometry. (b,c) Five weeks after VSV immunization, these mice were challenged intranasally with WT VSV (1 × 107 pfu/mouse). Six days after challenge, VSV-specific Abs and total Abs in lysate of brain (b) and serum (c) were measured by ELISA. Depletion of CD4 T cells was performed on −4, −1, 2 and 4 days after challenge by i.v. injection of anti-CD4 (GK1.5). (d) Albumin levels in tissue homogenates were analyzed by ELISA (Data are means ± s.e.m. *: p<0.05; *: p<0.01; ***: P<0.001 (Mann-Whitney U-test)).