Figure 4.
Gene expression after treatment of cultured tendon-derived stromal cells with IFNγ, LPS or IL13 for 96 hours. Tendon stromal cells were derived from healthy hamstring or diseased supraspinatus tendons (n=6 donors for LPS and IL13 treatment, n=5 donors for IFNγ treatment). (A) Treatment of cells from healthy hamstring (Ham) or diseased supraspinatus tendons (Supra) with 20 ngmL-1 IFNγ. (B) Stimulation of cells from healthy hamstring (Ham) or diseased supraspinatus tendons (Supra) with 100 ngmL-1 LPS. (C) Stimulation of cells from healthy hamstring (Ham) or diseased supraspinatus tendons (Supra) with 20ngmL-1 IL13. Statistically significant differences were calculated using pairwise Mann Whitney U tests. Gene expression is normalized to β-actin; bar represents median. *p<0.05, **p<0.01.