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. Author manuscript; available in PMC: 2016 May 27.
Published in final edited form as: Sci Transl Med. 2015 Oct 28;7(311):311ra173. doi: 10.1126/scitranslmed.aac4269

Figure 7.

Figure 7

The aspirin metabolite 15-epi LXA4 modulates inflammation in LPS-treated stromal cells derived from diseased tendons. Tendon cells were derived from intermediate stage disease supraspinatus tendons from a minimum of 5 patients. (A) Tendon-derived cells were treated with 100ngmL-1 LPS in medium containing 50nM 15-epi LXA4 for 96 hours. There was increased expression of CD206 mRNA after treatment with LPS and 15-epi LXA4 compared to LPS-alone (cells derived from tendons from 6 patients). (B,C,D) Tendon cells were treated with 100ngmL-1 LPS in medium containing 100nM 15-epi LXA4 for 96 hours. There was increased expression of ALOX15 mRNA (B) and CCL22 mRNA (C), and reduced expression of IL12B mRNA (D) compared to LPS-alone (cells derived from tendons from 5 patients). Statistically significant differences were calculated using pairwise Mann Whitney U tests. Gene expression is normalized to β-actin. Bar shows median values. * p<0.05, **p<0.01.