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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Acta Biomater. 2016 Mar 3;36:42–54. doi: 10.1016/j.actbio.2016.03.007

Fig. 7.

Fig. 7

In situ temporal stiffening regulates HAVIC osteogenic differentiation. (A) Compaction ratio of HAVIC laden hydrogels with different initial stiffness and in situ stiffening (upper images are representative hydrogel images at day 21); (B) compressive stiffness of 1 kPa, 3 kPa and in situ-stiffened hydrogels at day 7 and 21 (day 7 indicates the time point for in situ-stiffening, and day 21 indicates 14 days after in situ-stiffening); temporal stiffening process decreases ALP activity (C) and downregulated gene expressions of ALP and OCN (D) compared to 1 kPa hydrogels at day 21 in OGM (*p<0.05, **p<0.01; relative gene expression is presented as normalized to 18s and expressed relative to 1 kPa hydrogel in OGM).