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. 2012 Aug 31;5:23. doi: 10.1186/1939-8433-5-23

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© Andrieu et al.; licensee Springer. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of HpRNA T-DNA stable transformants. (A) Northern blot detection of OsPDS and OsSLR1 mRNA accumulation in different tissues of wild type plants of cv. Nipponbare. (B) Phenotypes of RNAi mutant lines expressing OsPDS RNAi HpRNA construct. (C) Detection of 21-nts siRNAs in OsPDS and OsSLR1 HpRNA transgenic lines exhibiting or not a mutant phenotype. A probe specific to the gene specific tag (GST) and a probe representing regions extending 3’ and 5’ from the GST interval have been sequentially used. L (leaf), R (root), Nb (Control Nipponbare plant).

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