Table 2.
Comparisons of the PCA results from the differentially expressed metabolites data of 15 amino acids (AAs), 31 organic acids (OAs) and 38 sugars and data of 918 related differentially expressed genes (DEGs) detected in LTH and IR29 under the chilling stress and non-stress control at the seedling stage
| Principal components (PC) | Metabolites or related DEGs1 | Transcriptomic data | Metabolomic data | ||
|---|---|---|---|---|---|
| N | % | N | % | ||
| PC1 (Stress treatments) | R2 (%) | 39.4 | 20.9 | ||
| AA+ | 140 | 37.5 | 1 | 6.7 | |
| AA- | 181 | 48.5 | 8 | 53.3 | |
| (AA+OA+sugar)+ | 94 | 32.2 | |||
| (AA+OA+sugar- | 143 | 49.0 | |||
| OA+ | 39 | 33.3 | 25 | 80.6 | |
| OA- | 53 | 45.3 | 0 | 0 | |
| (OA+sugar)+ | 7 | 13.7 | |||
| (OA+sugar)- | 28 | 54.9 | |||
| Sugar+ | 30 | 35.3 | 29 | 76.3 | |
| Sugar- | 33 | 38.8 | 0 | 0 | |
| PC3/PC2 (Genotypic differences) | R2 (%) | 13.5 | 13.8 | ||
| AA+ | 118 | 31.6 | 8 | 53.3 | |
| AA- | 60 | 19.2 | 2 | 13.3 | |
| (AA+OA+sugar)+ | 72 | 24.7 | |||
| (AA+OA+sugar- | 66 | 22.6 | |||
| OA+ | 36 | 30.8 | 7 | 22.6 | |
| OA- | 29 | 24.8 | 17 | 54.8 | |
| (OA+sugar)+ | 24 | 47.1 | |||
| (OA+sugar)- | 7 | 13.7 | |||
| Sugar+ | 29 | 34.1 | 16 | 42.1 | |
| Sugar- | 15 | 17.6 | 12 | 31.6 | |
| PC2/PC3 (Genotypic difference in recovery/treatment timing) | R2 (%) | 20.3 | 13.5 | ||
| AA+ | 108 | 29.0 | 13 | 86.7 | |
| AA- | 102 | 27.3 | 0 | 0 | |
| (AA+OA+sugar)+ | 81 | 27.7 | |||
| (AA+OA+sugar- | 97 | 33.2 | |||
| OA+ | 43 | 36.8 | 11 | 35.5 | |
| OA- | 35 | 29.9 | 5 | 16.1 | |
| (OA+sugar)+ | 24 | 47.1 | |||
| (OA+sugar)- | 16 | 31.4 | |||
| Sugar+ | 29 | 34.1 | 18 | 47.4 | |
| Sugar- | 33 | 38.8 | 9 | 23.7 | |
1 N is the number of related DEGs or metabolites showing significant positive (+) or negative (-) loading (contributions) to the corresponding principal components (PCs).