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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Immunother. 2016 Apr;39(3):105–116. doi: 10.1097/CJI.0000000000000116

Figure 3.

Figure 3

Recognition of WT1 peptide-pulsed tumor cell lines by PBL retrovirally transduced to express a WT1 reactive TCR. PBL from 3 HLA-A*0201+ donors were transduced with retroviral particles encoding GFP as a negative control or with the WT1 reactive TCR (TRAV12-1*01; CDR3: CVVNTPPNTDKLIF; TRBV7-2*01; CDR3: CASTPFTSGSGWDEQFF). A-C. Transduced T cells were cocultured overnight with tumor cell lines pre-pulsed with 1 μg/ml of WT1 peptide or in the absence of exogenously pulsed peptide, and IFNγ in coculture supernatants was measured by ELISA.