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. 2012 Dec 11;5:35. doi: 10.1186/1939-8433-5-35

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© Wamaitha et al.; licensee Springer. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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OsRap2.6 Ox plants are resistant to a compatible race (007) of M. oryzae. OsRap2.6 mRNA transcript levels from three independent Ox plants (P1, P6 and P14) were measured by qPCR. The Ox plants were grown in the greenhouse for two months and inoculated with M. oryzae compatible (virulent) Ina 86–137 (Race 007) fungal spore suspension. (A) Expression levels of OsRap2.6 transcripts in T1 OsRap2.6 Ox plants before infection with rice blast fungus. (B) Photographs showing lesions in leaf blades in WT and OsRap2.6 Ox plants after infection. (C) Quantitative analysis of fungal growth showing increased resistance in OsRap2.6 Ox plants 7 days after infection with rice blast fungus compatible race (007). Ubiquitin was used as an internal control. Bars represent the means ± SE calculated using four biological replicates where each replicate consists of three independent technical replicates (p ≤ 0.01, n=48). (D) Lesion length of OsRap2.6 Ox plants showing increased resistance to blast fungus as compared to WT as shown by increased lesion length after infection (p ≤ 0.01, n=48). (E) Expression of PAL1 mRNA in OsRap2.6 Ox plants after infection with the compatible race 007. Levels of PAL1 mRNA were up regulated as measured by reverse transcription qPCR (p ≤ 0.01, n=48).