Fig 4. Top1 deletion does not efficiently unsilence the paternal Ube3a allele.

(A) Schematic of the experimental setup used to assess Ube3a unsilencing. (B,C) Representative immunoblots and quantification of indicated protein levels normalized to ACTIN in neurons from Ube3a^m-/p+::Top1^wt/fl mice (B) or Ube3a^m-/p+::Top1^fl/fl mice (C). Mean ± s.e.m., unpaired student’s t-test; * p < 0.05, n = 3–4 cultures.