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. 2016 May 27;12(5):e1006062. doi: 10.1371/journal.pgen.1006062

Fig 2. ube3a regulates NMJ synapse growth.

Fig 2

(A) Muscle 4 NMJ synapses were double-stained with anti-HRP (green) and anti-CSP (magenta). The precise excision line P95 was used as the wild-type control. ube3a35 homozygotes showed more total boutons and satellite boutons (indicated by arrows) than wild-type larvae. A copy of wild-type genomic ube3a transgene (g+) rescued the NMJ phenotype of ube3a35 mutants, however, a copy of ube3a transgene harboring the missense mutation C941A (gC941A) did not. elav-Gal4-driven Thu3266 RNAi against ube3a in presynaptic neurons led to increased numbers of total and satellite boutons, while C57-Gal4-driven postsynaptic knockdown of Ube3a had no effect on NMJ growth. Scale bar = 10 μm. (B, C) Quantification of total bouton (B) and satellite bouton (C) number in different genotypes including wild type, ube3a35, genomic ube3a+/+; ube3a35, genomic ube3aC941A/+; ube3a35, elav-Gal4/+; RNAi/+, and RNAi/+; C57-Gal4/+. n ≥ 18 NMJs; **P < 0.01, ***P < 0.001; ns, not significant. Error bars represent SEM.