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. 2016 May 27;12(5):e1006062. doi: 10.1371/journal.pgen.1006062

Fig 4. ube3a interacts genetically with BMP signaling pathway components tkv and mad in regulating NMJ growth.

Fig 4

(A) Confocal images of muscle 4 NMJ synapses co-stained with anti-CSP (magenta) and anti-HRP (green) to reveal synaptic vesicles and presynaptic membrane, respectively. In ube3a tkv double mutants, bouton numbers were similar to those of tkv8/tkvK16713 single mutants. One copy of tkv8 rescued the synapse overgrowth in ube3a35 mutants. Scale bar = 10 μm. (B, C) Quantification of total bouton number (B) and satellite bouton number (C) of various genotypes including wild type, ube3a35, tkv8/tkvK16713, tkv8/ tkvK16713; ube3a35, tkv8/+, tkv8/+; ube3a35, mad12/+, mad12/+; ube3a35. n ≥ 18 NMJ terminals, one-way ANOVA test, ***P < 0.001, ns, not significant,. Error bars represent SEM.