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. 2016 May 27;11(5):e0156293. doi: 10.1371/journal.pone.0156293

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© 2016 Hughes et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A, Viability of vaginal T cells (indicated “CD3”) and macrophages (indicated “CD14”) following cryopreservation with 1.5M glycerol, 1.5M propylene glycol (PG) or 10% dimethyl sulfoxide (DMSO). B, Effect of CPA concentration on cell viability after cryopreservation. C, Effect of trehalose concentration on cell viability after cryopreservation in the presence 0.84M DMSO, 1.5M PG, or 1.75M EG. D, Effect of CPA concentration on cell viability after cryopreservation in the presence of 4.8% trehalose. E, Effect of trehalose concentration on recovery after cryopreservation in the presence of 1.4M DMSO. F, Effect of mixtures of DMSO and EG on cell recovery after cryopreservation in the presence of 1.2% trehalose. Each line for a given color indicates a different tissue sample. The line with the circles indicates the mean across the different tissue samples. Measurements were made in duplicate for each tissue sample. For relative viability, 100% indicates the same viability as the fresh cells.