Table 1. Overview of the three different cryopreservation procedures used.

Procedure	A	B	C: HANC
CPA addition before freezing	3 min, 4°C	3 min, 4°C	All at once, 4°C
Thawing	37°C water bath	37°C water bath	37°C water bath
CPA removal after thawing	3 min, 4°C	30s, 37°C	30s, 37°C
Benzonase in dilution medium	0	0	50U/mL
Washing volume	5 mL	10 mL	20 mL
Washing tube size	5 mL	15 mL	50 mL
Washes	1	1	2