Figure 2.

AvrBs1 attenuates activation of the yeast HOG pathway. Activation of an 8xCRE-regulated lacZ reporter monitored upon incubation of yeast strains containing an empty vector or the indicated effectors with or without 1 M sorbitol. Activity was normalized to that of yeast containing an empty vector and treated with sorbitol. Values represent the means ±SE of the relative activation 1 h after sorbitol addition in 3 biological repeats. Asterisks indicate a significant difference (Student's t test, P < 0.05) as compared to yeast containing an empty vector and treated with sorbitol.