Figure 2.

GID1 promote the dissociation of GAF1 from DELLA in GA-dependent manner. GAF1 fused with the GAL4 DNA-binding domain, GAI and RGA fused with the GAL4 activation domain and resulting β-galactosidase activity was detected in the presence or absence of GID1 and with or without 10⁻³ M GA₃. To avoid the suppression of transactivation activity of DELLAs by the masking of GA-GID1 to DELLA motif in yeast, we use GAL4 activation domain fused to DELLAs as prey protein. GAF1 interact with DELLAs, GAI and RGA, in yeast. The binding of GID1 promote the dissociation of GAF1 from DELLA in presence GA condition. Data are means±SD, n = 3 “vec” indicates empty vector using as negative control. Asterisks represent Student's t test significance compared with BD-GAF1 (*P < 0.05).