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. 2015 Aug 3;10(9):e1060385. doi: 10.1080/15592324.2015.1060385

Figure 1.

Figure 1.

Characterizations of antibodies against RanGAP and MAP65. (A) Schematic diagram of the domain structure of an onion RanGAP, AcRanGAP. AcRanGAP contains tryptophan-proline-proline domain (WPP), leucine-rich repeat ribonuclease inhibitor (LRR_RI)-like domain, and acidic domain (Acidic). Numbers represent amino acid positions, starting at the N-terminus. A peptide of AcRanGAP fragment shown as a bar (amino acid 1 to 497) in the figure was used as an antigen to produce antibody against AcRanGAP. (B) Immunoblot analysis of an antibody against AcRanGAP fragment in crude extract from onion root tips. Lane 1, Coomassie brilliant blue staining; lane 2, control immunoblot with a preimmune rabbit serum; lane 3, immunoblot with an affinity purified antibody against AcRanGAP; lane 4, control immunoblot with a mixture of a purified antibody against AcRanGAP preincubated with an antigen of AcRanGAP fragment. (C) Immunoblot analysis of antibody against tobacco BY2MAP65 in crude extract from onion root tips. Lane 1, Coomassie brilliant blue staining of a crude extract from onion root tips; lane 2, immunoblot with a mouse monoclonal antibody against tobacco BY2MAP65. (M) indicates the position of molecular mass markers and their molecular masses (kDa) in (B) and (C).