Localization of RanGAP and tubulin in onion root tip cells. Fluorescence images of chromosomes, RanGAPs and tubulins in onion root tip cells of interphase (A-C), mid-prophase (D-I), late prophase (J-L), prometaphase/metaphase transition (M-O), and telophase (P-R). A-C, D-F, G-I, J-L, M-O, and P-R are images of the same cells, respectively. Chromosomes are shown as white (A, D, G, J, M, P), RanGAP images are shown as green (B, E, H, K, N, Q) and tubulin as red (C, F, I, L, O, R). Asterisks indicates RanGAP band. Arrowhead shows the concentration of RanGAP around kinetochore. Bar: 5 μm.