Figure 5.

Effects of MT band modification by oryzalin and/or latrunculin B (LatB) on the localization of RanGAP and tubulin in root tip cells of 4-day-old onion seedlings 7 h after their transfer from 5-AU to distilled water. (A-C), immunofluorescence images of an onion root tip cell in mid-prophase treated with 20 µM oryzalin for 15 min. (D-F), immunofluorescence images of an onion root tip cell in late prophase treated with 20 µM oryzalin for 15 min. (G-I) immunofluorescence images of an onion root tip cell in late prophase treated with 10 µM Lat B for 15 min. A-C, and D-F are images of the same cells, respectively. Chromosomes are shown as white (A, D and G), RanGAP images are shown in green (B, E and I), and tubulin in red (C, F and I). Brackets indicate RanGAP and MT bands. Bar: 5 μm.