Figure 6.

Effects of MT band modification by cytoskeletal inhibitors on the relationship between width of MT bands and that of RanGAP bands in root tip cells of 4-day-old onion seedlings 7 h after their transfer from 5- AU to distilled water. Inhibitors were applied 15 min before chemical fixation. (A) Control experiment with 0.2% DMSO. (B) Root tips treated with 20 µM oryzalin for 15 min. (C) Root tips treated with 10 µM latrunculin B (LatB) for 15 min. The straight lines in each figure shows the points where the width of RanGAP band corresponds to that of MT band. The nuclear stages were determined by the Hoechest 33258 stainings. •, interphase cells; ◊, mid-prophase cells; ▴, late prophase cells.