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. 2016 Feb 6;11(4):e1139277. doi: 10.1080/15592324.2016.1139277

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Figure 1. — MAPKKK18 interacts with the SnRK2.6 kinase. (A) Pull-down assay to verify the interaction beteween SnRK2.6 and MAPKKK18. Input lines represent total SnRK2.6. Recombinant His- MAPKKK18, pre-coupled to Ni-NTA agarose, was incubated with recombinant GST-SnRK2.6. GST- and His-tagged proteins were detected (IB) using anti-GST and anti-His tag antibodies, respectively. (B) SnRK2.6 and MAPKKK18 proteins interact within the nucleus. Cytoplasmic interaction around the nucleus is present in about 10% of protoplasts. BiFC analysis in Arabidopsis protoplasts transiently expressing full-length SnRK2.6 and MAPKKK18 fused to cECFP or nVenus, respectively. RFPtag-CBP20 was used as both transformation control and marker of nuclear localization. Scale bar: 20 μm.