Effects of ABA on PP2A activity and formation of PP2A holoenzyme assembly. (A) ABA treatment and cold reduce PP2A activity in plant seedlings and seeds. MS, Murashige and Skoog medium; ABA 1 h, ABA 2 h, and ABA 24 h, plant seedlings were treated with ABA for 1, 2, and 24 hours, respectively. Bar indicates standard deviation. (B) Immunoprecipitation with PP2A-A antibodies brings down less PP2A-C subunits after ABA treatment. Proteins recognized by different antibodies are labeled on the right.