Figure 1.

M. truncatula DELLA proteins interact with RAD1. (A) Interaction between DELLA1/2 and RAD1 in a yeast-two hybrid assay. AD, LexA-activation domain; BD, LexA-binding domain. (B) HA-tagged RAD1 co-immunoprecipitates with YFP-tagged della2-Δ18. To increase sensitivity a GA-insensitive mutant protein of DELLA2 (della2-Δ18) was used. No HA-RAD1 protein was detected following immunoprecipitation with GFP. Proteins were transiently co-expressed via agro-infiltration in N. benthamiana leaves. The methods were as described previously.³³.