Figure 9.

Changes in soluble sugar levels in colonies of the wild-type (WT) and atg5 mutants of Physcomitrella cultured in the dark.  Seven-day-old colonies of WT and atg5 mutants of Physcomitrella were cultured for 7 d in the dark. Soluble sugars were extracted from the colonies on days 0, 3, and 7. (A) Total amounts of sugars were measured by the anthrone method using glucose as standard. (B) Extracted sugars were separated by thin-layer chromatography and stained with sulfuric acid. In the lane M, authentic glucose and sucrose were separated. (C) Levels of glucose and sucrose in the colonies of WT and atg5 mutants were estimated by densitometry using ChemiDoc (Bio-Rad).