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Effects of casamino acid addition to culture medium on dark-induced senescence in protonemal colonies of Physcomitrella. Seven-day-old colonies of WT and atg5 mutants (atg5–1, atg5–2 and atg5–3) of Physcomitrella were cultured on nutrient-sufficient medium supplemented with 0.5% (w/v) casamino acids for 7 d As a control, senescence on nutrient-sufficient medium with no supplement is shown (Control). Bar, 4 mm.
