Table 2.
Growth on different carbon sources
C. glutamicumstrain | Carbon source concentration | Maximum OD600 | Growth rate (h−1) |
---|---|---|---|
CIT1(pEKEx3-xylAB) | Xylose: 15 g/L | 6 ± 1 | 0.03 ± 0.01 |
CIT1(pEKEx3-nagB) | Glucosamine: 10 g/L | 3 ± 1 | 0.02 ± 0.01 |
CIT1(pAMY) | Soluble starch: 10 g/L Glucose: 2.5 g/L | 9 ± 1 | 0.21 ± 0.01 |
CIT1(pEC-XT99A) | Soluble starch: 10 g/L Glucose: 2.5 g/L | 3 ± 1 | 0.10 ± 0.01 |
Fermentations were performed in CGXII minimal medium containing the respective carbon source and were supplemented by 750 μM L-arginine. 1 mM IPTG and 25 μg/ml kanamycin and spectinomycin were added. Values and error bars represent the mean and the standard error of triplicates.