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. 2014 Nov 11;4:82. doi: 10.1186/s13568-014-0082-3

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© Shibasaki et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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IL-6 (A) and PGE2 (B) production by affibody protein-introduced MH7A cells. MH7A cells were transfected with affibody proteins. After 24 h of culture, the cells were stimulated with TNF-α (100 ng/mL) and cultured for an additional 24-48 h. IL-6 and PGE2 levels were analyzed, and percent inhibition of IL-6 or PGE2 production was determined. Percent inhibition was calculated as follows: ([(IL-6 or PGE2 levels in affibody protein-introduced MH7A cells) - (IL-6 or PGE2 levels in MH7A cells introduced with standard peptides)]/([IL-6 or PGE2 levels in MH7A cells introduced with standard peptides]) × 100. Data represent the means × SD of three independent experiments. *p <0.05.